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To be certain frozen cells received from distinctive vials inside a Functioning cell financial institution produce reproducible benefits, scientists really should adhere towards the cryopreservation protocol strictly. Here are some protocols and technical tricks for freezing specific cell styles:

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Cryopreservation is really a process of utilizing reduced temperatures to preserve cells and tissues for long term use. This technique involves cooling cells to very low temperatures (-80˚C to -196˚C) and suspending their cellular metabolism, which preserves the cells for an indefinite length of time.

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Made under suitable cGMPs, mTeSR™ Additionally ensures the very best high quality and regularity for fundamental exploration in addition to for cell therapy and investigational new drug investigate programs. It is predicated on mTeSR™1 (Catalog #85850), the most generally revealed feeder-cost-free cell society medium for hPSCs.

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Contamination-totally free cells: Just before freezing, make sure the cells are wholesome and free of any microbial contamination.